CIL:36642, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:crystalloid, mitochondrion, trichocyst; Biological process:cytoplasm organization

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36642^* Cite

Description

Quick-freeze deep-etch image of a crystal. The contents of the crystal have condensed into paracrystalline planes that break into angular chunks when fractured. A portion of the P-fracture face of the membrane has many pits where IMPs have been removed. This resembles the P-face of the phagolysosome membrane or stage III of digestive vacuoles. TEM taken on 4/6/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: crystalloid; mitochondrion; trichocyst

Biological Context

Biological Process: cytoplasm organization

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36642
Archival Resource Key (ARK): ark:/b7295/w9cil36642

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2535px	——
Y	3000px	——