CIL:36653, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

NCBI Organism:Paramecium multimicronucleatum; Cell Types:cell by organism, eukaryotic cell, , ; Cell Components:micronucleus, microtubule associated complex; Biological process:nuclear division

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36653^* Cite

Description

The micronuclear separation spindle. Nuclear pores are not empty perforations but are closed by nonetchable material. The separation spindle must elongate as microtubules lengthen and/or slide against each other as they grow. TEM taken on 5/12/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm.

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: micronucleus; microtubule associated complex

Biological Context

Biological Process: nuclear division

Attribution

Names: Richard Allen (University of Hawaii)

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL36653
Archival Resource Key (ARK): ark:/b7295/w9cil36653

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Image Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop
Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Methods: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3014px	——
Y	3865px	——