Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 5 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 53 degrees. The image is grouped with one of the same sample area recorded at a 74 degree tilt. A stereo examination of the pair provides an oblique 3D view.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 5984px | 2.54µm |
Y | 4152px | 2.54µm |