A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged ESP1. ESP1 is essential for dissolving the cohesins that hold the sister chromatids together. ESP1 activation results in proper separation of sister chromatids at anaphase ESP1 also inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit. These phase, gfp z-series images are part of an image group that ranges from CIL:39566-39570. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 512px | 213.4nm |
Y | 512px | 213.4nm |
Time | 300 microns | 61 |
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Time | 0.5 microns | 21 |