Higher magnification view of the spongiome of the Contractile Vacuole Complex. The spongiome is composed of a three dimensional array of tubules that are continuous with the CV membrane. Many tubules bear peg-like elements on their cytosolic surfaces reminiscent of the decorated tubules of Paramecium. These pegs are probably V-ATPase proton pumps. TEM taken on 5/1/78 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 18,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Spatial Axis | Image Size | Pixel Size |
---|---|---|
X | 4837px | 0.83nm |
Y | 4532px | 0.83nm |