FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
1.1_Cell_1_and_Cell_6_Raw_Image_Stack is a multipage TIFF file consisting of 388 individual EM sections acquired at 15 nm milling step, resulting in 2.43×2.43×15 nm³ voxel size. This file also contains the raw data related to Cell#6. Note: The data acquisition session was interrupted after frame #161 to adjust the imaging area, resulting in a large shift on subsequent frame #162.
This file is a part of Dataset 1, comprised of files related to the Cell #1 of current study. The numbering order of the files reflects the order of operations performed during the analysis.