FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
1.4_Cell_1_Stack_Filtered is a multipage TIFF file filtered using mean shift (kernel: 3D, 3 > max distance X & Y: 3 > noise spread: 1.0), unsharp (unit: 8 > kernel: 3D, 3 > standard deviation: 1.0 > unsharp factor: 1.0), and histogram balancing (reference slide #170).
This file is a part of Dataset 1, comprised of files related to the Cell #1 of current study. The numbering order of the files reflects the order of operations performed during the analysis.