FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
2.6_Cell_2_GoldParticle_Segmentation is a multipage TIFF file illustrating the gold bead segmentation results (1.95×1.95×10 nm³ voxel size). Same as ‘GoldParticles.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview.
This file is a part of Dataset 2, comprised of files related to the Cell #2 of current study. The numbering order of the files reflects the order of operations performed during the analysis.