FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
4.5_Cell_4_Stack_Filtered is a multipage TIFF file consisting of 368 individual EM sections (1.95×1.95×15 nm³ voxel size) filtered with slope map filter (unit: 8 > scale: 10), unsharp filter (unit: 8 > kernel: 3D, 5 > standard deviation: 1.0 > unsharp factor: 0.0), and median filter (unit: 8 > kernel: 3D, cube, 3).
This file is a part of Dataset 4, comprised of files related to the Cell #4. The numbering order of the files reflects the order of operations performed during the analysis. Note: The data acquisition session was interrupted after frame #224, and later resumed as a separate imaging session. This resulted in two stack files, concatenated into a single multipage TIFF file during data analysis.