To determine what happens to the CV during systole we observed the CV in a living cell and then fixed the cell as soon as the CV had opened to the outside. It was apparent that the CV membrane does no...

A high resolution micrograph of the Contractile Vacuole Complex membrane system to determine what happens to the CV during systole we observed the CV in a living cell and then fixed the cell as soon a...

High resolution image of the decorated spongiome is composed of 50nm tubules that have a pattern of helically wound subunits attached to their cytosolic surfaces. These tubules can be up to one microm...

A partially filled contractile vacuole is connected to two regions of spongiome tubules. The extra CV membrane is acquired from these spongiomes. Presumably some of these spongiome tubules are decorat...

PINK1-YFP R98F (green) is localized to mitochondria in the absence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone). The R98F mutation in PINK1-YFP partially in...

YFP-WT PINK1 (green) shows mitochondrial localization in HeLa cells following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are...

Larger vesicles contain paracrystalline cores that are associated at one end with the trichocyst membrane. This may mean that there is a site on the membrane that promotes paracrystalline formation or...

Electron micrograph of an unmyelinated nerve Schwann cell from a rat tongue. Image made available by James D. Jamieson and the Department of Cell Biology, Yale University School of Medicine.

When trichocysts are triggered to discharge their contents the body of the trichocyst expands 8 or 9 times into a long needle like filament, however, the trichocyst tip does not change shape. The tric...

A live amoeba treated with the membrane-binding vital dye FM4-64 which stains the plasma membrane, contractile vacuole and other organelles. Large membrane-bounded organelles are excluded from the bro...